内容简介
张淑平、李鹏主编的《分子生物学实验指导(英文版)》以科研思路的培养为基本出发点，以真核生物基因为实验材料，涵盖DNA提取、分子克隆、蛋白质表达纯化、RNA提取、表达谱分析、转录水平调控分析方法等多个基础实验。各实验既可以单独开设，也可以将前后联系的独立实验整合为综合实验。每个实验分为背景知识与概念、操作步骤、注意事项以及思考题等内容，并在书后附仪器的使用说明、基本操作的介绍，网络资源使用简介等补充知识。全书双色印刷。
目录
Experiment 1
Preparation of Plasmid DNA
Experiment 2
Restriction Enzyme Digestion and Agarose Gel Electrophoresis
Experiment 3
Polymerase Chain Reaction(PCR)
Experiment 4
DNA Purification and Construction of Recombinant DNA
Experiment 5
Preparation of Competent E.coli Cells
Experiment 6
Transformation of Recombinant DNA
Experiment 7
Identification and Characterization of Positive Clones
Experiment 8
Isolation ofTotal RNA from Animal Tissues
Experiment 9
Molecular Hybridization
Experiment 10
Reverse Transcription—Polymerase Chain Reaction(RT-PCR) and TA-cloning
Experiment 11
Expression and Purification of a Fusion Protein from E.coli
Experiment 12
Electrophoretic Mobility Gel Shift Assay(EMSA)to Detect DNA—Protein Interactions
Appendix I
Brief Introduction to the NCBI Database
Appendix II
Vector Information
Appendix III
Instructions on How to Use the Equipment
References
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